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The 3′‐untranslated region of brome mosaic virus RNA does not enhancetranslation of capped mRNAs in vitro
Author(s) -
О. В. Карпова,
Vessela Mavrodieva,
O.L. Tomashevskaya,
Н. В. Родионова,
J.G. Atabekov
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00103-g
Subject(s) - brome mosaic virus , messenger rna , untranslated region , rna , five prime untranslated region , three prime untranslated region , reticulocyte , translation (biology) , in vitro , microbiology and biotechnology , gene , biology , virus , virology , chemistry , genetics , rna dependent rna polymerase
The translation enhancing ability of cis ‐acting 3′‐terminal untranslated region (3′‐UTR) of brome mosaic virus (BMV) was examined. Two chimeric mRNA constructs translated in rabbit reticulocyte lysates contained the BMV coat protein (CP) gene and NPTI gene, respectively. It was shown that the 3′‐UTR of BMV RNA enhanced the translational efficiency of uncapped but not capped messages.