Premium
Kinetics of the unfolding‐folding transition of Bacillus subtilis levansucrase precursor
Author(s) -
Pier A. Scotti,
Régis Chambert,
MarieFrançoise PetitGlatron
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00099-u
Subject(s) - levansucrase , bacillus subtilis , kinetics , chemistry , folding (dsp implementation) , transition (genetics) , protein folding , biophysics , crystallography , biochemistry , bacteria , biology , physics , genetics , quantum mechanics , gene , electrical engineering , engineering
The reversible folding‐unfolding transition of mature and precursor forms of Bacillus subtilis levansucrase were compared under physiological conditions of pH and temperature. The time constant of the folding reaction was not modified by the presence of the signal sequence and the precursor in the native form was slightly more resistant to the denaturing action of urea. However, the folding pathway could be different for each protein since a domain of the mature levansucrase underwent an independent transition which is not observed during the renaturation process of prelevansucrase.