Premium
Nitrite and nitric oxide reduction in Paracoccus denitrificans is under the control of NNR, a regulatory protein that belongs to the FNR family of transcriptional activators
Author(s) -
Van Spanning Rob J.M,
De Boer Anthonius P.N,
Reijnders Willem N.M,
Spiro Stephen,
Westerhoff Hans V,
Stouthamer Adriaan H,
Van der Oost John
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00091-m
Subject(s) - paracoccus denitrificans , nitrite reductase , biochemistry , nitric oxide , reductase , denitrification , nitrate reductase , nitrite , biology , chemistry , enzyme , nitrate , nitrogen , ecology , organic chemistry , endocrinology
The nir and nor genes, which encode nitrite and nitric oxide reductase, lie close together on the DNA of Paracoccus denitrificans . We here identify an adjacent gene, nnr , which is involved in the expression of nir and nor under anaerobic conditions. The corresponding protein of 224 amino acids is homologous with the family of FNR proteins, although it lacks the N‐terminal cysteines. A mutation in the nnr gene had a negative effect on the expression of nitrite and nitric oxide reductase. Synthesis of membrane bound nitrate reductase, of nitrous oxide reductase, and of the cbb 3 ‐type cytochrome c oxidase were not affected by mutation of this gene. These results suggest that denitrification in P. denitrificans may be governed by a signal transduction network that is similar to that involved in oxygen regulation of nitrogen metabolism in other organisms.