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Colony‐stimulating factors and interferon‐γ activate a protein related to MGF‐Stat 5 to cause formation of the differentiation‐induced factor in myeloid cells
Author(s) -
Barahmand-pour Fariba,
Meinke Andreas,
Eilers Andreas,
Gouilleux Fabrice,
Groner Bernd,
Decker Thomas
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00072-h
Subject(s) - stat , stat4 , biology , microbiology and biotechnology , stat protein , cytokine , transcription factor , jak stat signaling pathway , colony stimulating factor , signal transduction , chemistry , immunology , haematopoiesis , stat3 , biochemistry , gene , tyrosine kinase , stem cell
The Jak‐Stat pathway of intracellular signals is used by growth factor‐ and cytokine receptors to induce gene transcription. We have recently reported that differentiation of myeloid cells, induced by phorbol ester, interferon‐γ (IFN‐γ) or colony‐stimulating factor‐1 (CSF‐1) is accompanied by the activation of the differentiation‐induced factor (DIF). Activated DIF specifically associates with a subclass of gamma‐interferon activation site (GAS)‐like DNA elements. We now report that GM‐CSF, which like CSF‐1 promotes the generation of mature macrophages, activates DIF. No activation was observed after treatment with the granulocyte growth and differentiation factor G‐ CSF. Antibodies raised against a Stat family protein, designated mammary gland factor‐Stat 5 (MGF‐Stat 5), reacted with DIF induced by either CSF‐1, GM‐CSF or IFN‐γ. Antisera to other known Stats were without effect on the DIF complex in electrophoretic mobility shift assays (EMSA). A 112 kDa protein could be isolated from either GM‐CSF‐ or IFN‐γ‐treated cells by GAS oligonucleotide precipitation. This protein reacted with antibodies to both MGF‐Stat 5 and phosphotyrosine. MGF‐Stat 5 and closely related proteins thus define a subfamily of Stat transcription factors that are present in a variety of cell types and are required for the onset of immediate gene expression in response to differentiating stimuli.