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Extension of DNA triple helix formation to a neighbouring (AT) n site
Author(s) -
Chandler Simon P.,
Fox Keith R.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00069-l
Subject(s) - triple helix , footprinting , oligonucleotide , chemistry , manganese , ligand (biochemistry) , helix (gastropod) , stereochemistry , sequence (biology) , intermolecular force , crystallography , dna , binding site , base sequence , molecule , biology , biochemistry , ecology , receptor , organic chemistry , snail
We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A 11 (AT) 6 · (AT) 6 T 11 , using oligonucleotides designed to form parallel T · AT and G · TA triplets. We find that, although (TG) 6 does not form a complex with (AT) 6 · (AT) 6 , T 11 (TG) 6 forms a stable structure producing a clear footprint which includes the (AT) 6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex‐binding ligand.