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Electrogenic Na + transport by Enterococcus hirae Na + ‐ATPase
Author(s) -
Kakinuma Yoshimi,
Igarashi Kazuei
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00056-f
Subject(s) - enterococcus hirae , extracellular , atpase , chemistry , mutant , intracellular , sodium , biophysics , v atpase , biochemistry , enzyme , biology , enterococcus , organic chemistry , gene , antibiotics
Energy‐dependent generation of a membrane potential (Δψ) (−45 mV, interior negative) was observed in the F 0 F 1 , H + ‐ATPase‐defective mutant of Enterococcus hirae . The generation of Δψ was found at high pH (but not at low pH), for which intracellular Na + was required but not extracellular K + . The Δψ‐generating activity was induced in cells cultured in media containing high concentrations of Na + , and was not observed in the Na + ‐ATPase mutants. These results suggest that E. hirae Na + ‐ATPase is responsible for the electrogenic sodium pump.