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Low frequency MFs increased inositol 1,4,5‐trisphosphate levels in the Jurkat cell line
Author(s) -
Korzh-Sleptsova I.L.,
Lindström E.,
Hansson Mild K.,
Berglund A.,
Lundgren E.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00031-4
Subject(s) - jurkat cells , inositol , line (geometry) , chemistry , microbiology and biotechnology , cell culture , biophysics , biology , receptor , biochemistry , immunology , t cell , genetics , mathematics , immune system , geometry
We have earlier reported that when a weak 50 Hz MF (magnetic field) was applied, the leukemic T‐cell line Jurkat responded with intracellular calcium oscillations [Lindström, et al., J. Cell Physiol ., 156 (1993) 395–398]. The result suggested that the MF interfered with the signal transduction, although neither target molecules nor molecular mechanisms are at present known. In this study we found that application of a MF to Jurkat cells resulted in significant increase of inositol 1,4,5‐trisphosphate (IP 3 ) levels. Chelation of intracellular calcium ions by BAPTA/AM, did not block the increase of IP 3 induced by MF. This result implied that MF‐induced Ca 2+ oscillations were not due to direct stimulation of the Ca 2+ ‐dependent phospholipase C‐γ1 (PLC‐γ1).