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Differentiation‐inducing factor of D. discoideum raises intracellular calcium concentration and suppresses cell growth in rat pancreatic AR42J cells
Author(s) -
Kubohara Yuzuru,
Saito Yutaka,
Tatemoto Kazuhiko
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00022-2
Subject(s) - dictyostelium discoideum , extracellular , intracellular , calcium , microbiology and biotechnology , calcium in biology , chemistry , cell growth , morphogen , growth factor , biology , biophysics , biochemistry , receptor , organic chemistry , gene
DIF (differentiation‐inducing factor) is a putative morphogen that induces stalk cell differentiation in the lower eukaryote, Dictyostelium discoideum . In this study, we have examined the effects of DIF on growth and the intracellular calcium concentration ([Ca 2+ ] i ) in rat pancreatic acinar AR42J cells. Growth of AR42J cells was inhibited when DIF was present in the media, and approximately 50% growth inhibition was attained with 20 μM DIF. DIF was also found to raise [Ca 2+ ] i in a dose‐dependent manner (1–40 μM), both in the presence and absence of extracellular Ca 2+ . These results suggest that DIF elicits both calcium influx from the extracellular space and calcium release from intracellular pool(s), thereby inhibiting cell growth in AR42J.