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Viral Qβ RNA as a high expression vector for mRNA translation in a cell‐free system
Author(s) -
Katanaev Vladimir L.,
Kurnasov Oleg V.,
Spirin Alexander S.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00006-u
Subject(s) - cistron , messenger rna , translation (biology) , rna , microbiology and biotechnology , dihydrofolate reductase , protein biosynthesis , rna dependent rna polymerase , ribosome , escherichia coli , biology , chemistry , virology , enzyme , biochemistry , gene
Dihydrofolate reductase (DHFR) mRNA was inserted into Qβ phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell‐free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Qβ replicase as compared with its synthesis when the original Qβ RNA was used.