Premium
Isolation and characterization of a gene for a ryanodine receptor/calcium release channel in Drosophila melanogaster
Author(s) -
Takeshima Hiroshi,
Nishi Miyuki,
Iwabe Naoyuki,
Miyata Takashi,
Hosoya Toshihiko,
Masai Ichiro,
Hotta Yoshiki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80634-9
Subject(s) - biology , polytene chromosome , ryanodine receptor , drosophila melanogaster , microbiology and biotechnology , gene , complementary dna , exon , genetics , calcium channel , nucleic acid sequence , receptor , calcium , chemistry , organic chemistry
The nucleotide sequence of a 25.7 kilobase Drosophila melanogaster genomic DNA segment containing a gene for a ryanodine receptor/calcium release channel homologue has been determined. Computer analysis and partial cDNA cloning revealed 26 exons comprising the protein‐coding sequence in this gene. The predicted protein is homologous in amino acid sequence and shares characteristic structural features with the mammalian ryanodine receptors. In blot hybridization analysis, a ~16 kilobase RNA species was identified abundantly in a 6–12 h embryo as the transcript from this gene. In situ hybridization to polytene chromosomes indicated that this gene locates at band position 44F on the second chromosome.