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A novel plant glutathione peroxidase‐like protein provides tolerance to oxygen radicals generated by paraquat in Escherichia coli
Author(s) -
Holland Doron,
Faltin Zehava,
Perl Avi,
Ben-Hayyim Gozal,
Eshdat Yuval
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80628-4
Subject(s) - paraquat , radical , escherichia coli , peroxidase , biochemistry , chemistry , glutathione , bacteria , glutathione peroxidase , antioxidant , enzyme , microbiology and biotechnology , biology , genetics , gene
Citrus salt‐stress associated protein (Cit‐SAP) reveals significant sequence homology to mammalian glutathione peroxidase (GP). In an attempt to assign biological function to this protein, transformed E. coli cells expressing Cit‐SAP were examined for their ability to tolerate free radicals formed by paraquat, an O − 2 radical forming agent. In the presence of paraquat, the survival rate of the transformed bacteria expressing Cit‐SAP was much higher as compared to the wild‐type bacteria. The results support the assumption that Cit‐SAP is a plant GP‐like protein which participate in the enzymatic system aimed at scavenging oxygen free‐radicals in plants.