Premium
Limited diffusibility of gene products directed by a single nucleus in the cytoplasm of multinucleated myofibres
Author(s) -
Ono Tomoko,
Ono Katsuhiko,
Mizukawa Kiminao,
Ohta Tetsuya,
Tsuchiya Tomofusa,
Tsuda Masaaki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80621-7
Subject(s) - nucleus , cytoplasm , beta (programming language) , microbiology and biotechnology , chemistry , biology , gene , biochemistry , computer science , programming language
Two types of β‐galactosidase genes, whose products are distributed in the nucleus (Nα‐gal) or cytoplasm (Cβ‐gal), were injected with fructose intramuscularly into the quadriceps of adult mice. Regionally restricted and overlapped distributions of both gene products were observed in the myofibres. These findings indicate that Nβ‐gal is incorporated into the nucleus responsible for its synthesis and that Cβ‐gal becomes located in the vicinity of the nucleus after its synthesis. This restricted location of C β‐gal in myofibres remained unchanged during the development of infant mouse muscle. Thus, the gene products directed by the nucleus of myofibres seem to show limited diffusibility, suggesting a universal localization of subcellular domains in myofibres.