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Regulation of triacylglycerol synthesis in permeabilized rat hepatocytes
Author(s) -
Stals Hedwig K.,
Top W.,
Declercq Peter E.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80615-2
Subject(s) - biochemistry , fatty acid , enzyme , fatty acid synthesis , biology , chemistry
Isolated hepatocytes from fed and starved rats, permeabilized with Staphylococcus aureus α‐toxin, were incubated with increasing concentrations of radiolabelled fatty acids, in the presence of a saturating concentration of 3‐GP. Incorporation of label into LPA, PA and DAG was lower in cells from starved rats than in cells from fed rats, apparently reflecting the lower activity of GPAT after starvation. This enzyme approached saturation at high fatty acid levels and determined the overall flux through the esterification pathway. TAG synthesis, however, was the same in both nutritional states and could not be saturated with fatty acid under the given conditions. Taken together with the observed accumulation of DAG, these data suggest that the rate of TAG synthesis is controlled by the fatty acid supply and, more particularly, by the affinity of DGAT for acyl‐CoA.