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Collagen‐binding recombinant fibronectin fragments containing type II domains
Author(s) -
Skorstengaard Karua,
Holtet Thor L.,
Etzerodt Michael,
Thøgersen Hans C.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80604-7
Subject(s) - fibronectin , recombinant dna , chemistry , cleavage (geology) , fusion protein , escherichia coli , type ii collagen , fragment (logic) , type iv collagen , biochemistry , microbiology and biotechnology , biology , extracellular matrix , gene , laminin , paleontology , cartilage , anatomy , fracture (geology) , computer science , programming language
Each of the two type II domains and four larger fragments, containing one or two type II domains of fibronectin, have been expressed in Escherichia coli . A special vector, containing a fragment encoding the cleavage site for Factor X a , Ile‐Glu‐Gly‐Arg, inserted immediately before the protein fragment of interest, was used. After treatment of the purified fusion proteins with reduced/oxidized glutathione, the correctly folded fibronectin fragments were released by proteolytic digestion with Factor X a . The largest fragment, consisting of two type II and two type I domains, was the only fragment able to bind to immobilized gelatin.