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cDNA cloning and regional distribution of a novel member of the opioid receptor family
Author(s) -
Fukuda Kazuhiko,
Kato Shigehisa,
Mori Kenjiro,
Nishi Miyuki,
Takeshima Hiroshi,
Iwabe Naoyuki,
Miyata Takashi,
Houtani Takeshi,
Sugimoto Tetsuo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80603-9
Subject(s) - complementary dna , microbiology and biotechnology , cdna library , opioid receptor , in situ hybridization , biology , northern blot , messenger rna , orphan receptor , δ opioid receptor , receptor , gene , genetics , opioid , transcription factor
We have cloned a cDNA for a novel member of the opioid receptor family, designated as ROR‐C, from the rat cerebrum cDNA library using the probe derived from the δ‐opioid receptor subtype cDNA. The deduced amino acid sequence of ROR‐C shows high homology with those of ROR‐A (rat δ‐opioid receptor subtype), ROR‐B (rat μ‐subtype) and ROR‐D (rat k ‐subtype). RNA blot hybridization and in situ hybridization analysis revealed that ROR‐C mRNA is expressed in discrete regions of the rat central nervous system.