Premium
Cloning and functional characterization of the human 5‐HT 2B serotonin receptor
Author(s) -
Schmuck Karin,
Ullmer Christoph,
Engels Peter,
Lübbert Hermann
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80590-3
Subject(s) - 5 ht5a receptor , receptor , interleukin 21 receptor , 5 ht7 receptor , gabbr1 , complementary dna , microbiology and biotechnology , biology , protease activated receptor 2 , cloning (programming) , hek 293 cells , expression cloning , enzyme linked receptor , 5 ht receptor , clone (java method) , estrogen related receptor gamma , cdna library , exon , genetics , serotonin , gene , nuclear receptor , computer science , transcription factor , programming language
Recently, we have reported the cloning of the rat 5‐HT 2B receptor cDNA. This receptor is particularly interesting since it may be involved in diseases such as migraine. Here, we describe the isolation of a human 5‐HT 2B receptor clone from a cDNA library derived from SH‐SY5Y cells. Although the receptor sequence was only 80% homologous to the rat sequence, the exon‐intron distribution was conserved between the two species. In the human body, the receptor mRNA was detected in most peripheral organs. Only low expression levels were found in the brain. After expression in HEK 293 cells, activation of the receptor stimulated the production of phosphatidylinositol. The pharmacology of this functional response correlated well with that of the rodent receptor.