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Regulatory sequences required for hst‐1 expression in embryonal carcinoma cells
Author(s) -
Koda Toshiaki,
Hasan Shahid,
Sasaki Akio,
Arimura Yutaka,
Kakinuma Mitsuaki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80587-3
Subject(s) - hela , enhancer , transcription factor , embryonic stem cell , embryonal carcinoma , untranslated region , biology , microbiology and biotechnology , cellular differentiation , transcription (linguistics) , gene , promoter , gene expression , cell culture , chemistry , messenger rna , genetics , linguistics , philosophy
The hst‐1 gene, which is implicated in mammalian embryonic development and morphological transformation of NIH3T3 cells, is expressed in undifferentiated F9 cells, but not in differentiated F9 and other well‐differentiated cells, such as PYS‐2, NIH3T3 and HeLa cells. An octamer element present in the 3' untranslated region acts as an enhancer. Although Oct3 is down‐regulated when F9 cells are differentiated, transient expression of Oct3 did not enhance the hst‐1 promoter activity in HeLa, NIH3T3 or PYS‐2 cells. Thus, the role of Oct3 on hst‐1 expression remains elusive, and an additional transcription factor which interacts may regulate hst‐1 transcription in association with Oct1, Oct3 or both.