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The primary structure of two chlorosome proteins from Chloroflexus aurantiacus
Author(s) -
Niedermeier Georg,
Shiozawa Judith A.,
Lottspeich Friedrich,
Feick Reiner G.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80585-7
Subject(s) - chlorosome , protein primary structure , nucleic acid sequence , biology , rna , nucleotide , amino acid , sequence (biology) , gene , peptide sequence , genetics , microbiology and biotechnology , chemistry , biochemistry , bacteriochlorophyll , bacteria
The complete nucleotide sequence of two chlorosome proteins with apparent molecular weights of M r 18,000 and M r 11,000 from Chloroflexus aurantiacus have been determined. The two polypeptides were 145 and 97 amino acids long and possessed true molecular masses of 15,545 and 10,820 Da, respectively. Protein chemical sequencing was done in parallel to confirm the primary structure deduced from nucleotide sequencing. By Northern blot analysis of RNA isolated from phototrophically grown cells a transcript of 0.95 kb was detected which is the expected length for a mRNA encoding both genes.