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Peroxynitrite releases copper from caeruloplasmin: implications for atherosclerosis
Author(s) -
Swain Jason A.,
Darley-Usmar Victor,
Gutteridge John M.C.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80582-2
Subject(s) - ceruloplasmin , peroxynitrite , copper , chemistry , nitric oxide , biochemistry , enzyme , superoxide , organic chemistry
Peroxynitrite may be formed in the vasculature by the reaction of puperoxide with nitric oxide. When the blue copper‐containing protein, caeruloplasmin, is incubated with peroxynitrite, copper is released, and ferroxidase activity and the blue colouration are lost. When plasma from normal subjects is incubated with peroxynitrite, the oxidant reacts with numerous plasma constituents but is still able to release copper from caeruloplasmin. As the ferroxidase activity of caeruloplasmin is lost in plasma in the presence of peroxynitrite, a second ferroxidase activity associated with peroxidised lipids, and not inhibited by azide, is formed.