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Expression of α‐1,3 linkage‐containing oligomannosyl residues in a cell‐wall mannan of Candida tropicalis grown in yeast extract‐Sabouraud liquid medium under acidic conditions
Author(s) -
Kobayashi Hidemitsu,
Shibata Nobuyuki,
Suzuki Akifumi,
Takahashi Shin-ichi,
Suzuki Masako,
Matsuda Kyoko,
Hisamichi Kanehiko,
Suzuki Shigeo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80576-8
Subject(s) - candida tropicalis , yeast , mannan , chemistry , hydrophobin , cell wall , liquid medium , biochemistry , microbiology and biotechnology , saccharomyces cerevisiae , biology , chromatography , gene , polysaccharide
We investigated the cell‐wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract‐Sabouraud medium at pH 3.0 by two‐dimensional homonuclear Hartmann‐Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a β‐1,2‐linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of α‐1,3 linkage‐containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of α‐1,3‐linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Man p α1‐3Man p α1‐2Man p α1‐2Man p α1‐2Man, and Man p α1‐2Man p α1‐3Man p α1‐2Man p α1‐2Man p α1‐2Man, in the mannan from cells grown at pH 3.0.