Premium
Rapid validation of molecular structures of biological samples by electrospray‐mass spectrometry
Author(s) -
Ashton D.S.,
Beddell C.R.,
Green B.N.,
Oliver R.W.A.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80572-5
Subject(s) - mass spectrometry , chemistry , electrospray ionization , electrospray mass spectrometry , electrospray , macromolecule , molecular mass , chromatography , molecule , analytical chemistry (journal) , biochemistry , organic chemistry , enzyme
A short account is presented of the method of measuring molecular masses ( M r ) of pure biological samples by electrospray ionisation mass spectrometry. It is demonstrated that the technique yields M r values with an effective accuracy equal to or better than 0.008% of the calculated M r , provided that the correct molecular structure is employed in the calculation. It is therefore recommended that this method of measuring M r 's should be considered to form an essential part of all studies aimed at elucidating the molecular structure of purified biological macromolecules or for confirming the identity of labelled samples of such molecules.