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Influenza virus M2 protein modifies membrane permeability in E. coli cells
Author(s) -
Guinea Rosario,
Carrasco Luis
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80564-4
Subject(s) - semliki forest virus , lysis , virus , escherichia coli , vesicle associated membrane protein 8 , biology , bacteria , integral membrane protein , membrane protein , membrane , microbiology and biotechnology , chemistry , biochemistry , virology , rna , gene , genetics
The M2 protein of influenza virus is an integral membrane protein with ion channel activity. This protein has been expressed in E. coli cells in an inducible manner. Expression of the M2 protein causes rapid lysis of BL21(DE3) pLysS E. coli cells upon induction with IPTG. M2 protein increases membrane permeability to a number of hydrophylic molecules, such as ONPG, uridine or impermeant translation inhibitors. The behaviour of M2 in bacteria resembles that of other viral proteins, such as poliovirus 3A and Semliki Forest virus 6K.