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A well resolved ODMR triplet minus singlet spectrum of P680 from PSII particles
Author(s) -
Carbonera Donatella,
Giacometti Giovanni,
Agostini Giancarlo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80555-5
Subject(s) - p680 , photosynthetic reaction centre , dimer , chemistry , photosystem ii , photochemistry , laser linewidth , singlet state , exciton , atomic physics , physics , excited state , electron transfer , photosynthesis , photosystem i , optics , condensed matter physics , laser , biochemistry , organic chemistry
An ADMR T‐S spectrum of the primary donor (P680) of photosystem II (PSII) was obtained from anaerobically photoreduced particles. The spectrum is the best resolved obtained so far having a main bleaching band at 684 nm with a linewidth of only 100 cm −1 . The view that this spectrum is produced by native homogeneous P680 unlike those obtained before is defended. A small bleaching observed at 678 nm is discussed in terms of the reaction center structure. One possible interpretation of the observations is that P680 is a very loose dimer with an exciton splitting of only 144 cm −1 corresponding to a dimer center‐to‐center distance of roughly 11.5 Å.