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Formation of 22 and 24 carbon 6‐desaturated fatty acids from exogenous deuterated arachidonic acid is activated in THP‐1 cells at high substrate concentrations
Author(s) -
Caruso D.,
Risé P.,
Galella G.,
Regazzoni C.,
Toia A.,
Galli G.,
Galli C.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80554-7
Subject(s) - arachidonic acid , chemistry , substrate (aquarium) , fatty acid , deuterium , mass spectrometry , chromatography , stereochemistry , biochemistry , enzyme , biology , ecology , physics , quantum mechanics
Deuterated arachidonic acid (AA, [ 2 H 8 20 :4 n‐6) 1–25 μM, is converted to other fatty acids, as evaluated by gas chromatography‐mass spectrometry, in THP‐1 cells. The major products, in the 1 to 10 μM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 μM, 24:4, 24:5 and 22:5 accumulate, with [ 2 H 8 ]/[ 2 H 0 ] ratios higher than in AA. At high AA concentration preferential conversion to elongated fatty acids with 5 unsaturations, through a 6 desaturase takes place and the 4‐desaturated 22:5 appears to be formed through β‐oxidation of 24:5.