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HNF1α activates the aminopeptidase N promoter in intestinal (Caco‐2) cells
Author(s) -
Olsen Jørgen,
Laustsen Liselotte,
Troelsen Jesper
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80525-3
Subject(s) - caco 2 , aminopeptidase , electrophoretic mobility shift assay , transfection , chemistry , microbiology and biotechnology , biochemistry , gene , gene expression , biology , in vitro , leucine , amino acid
The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco‐2 cell‐line. Aminopeptidase N promoter activity in Caco‐2 cells depends on the HNF1 element (positions −85 to −58) and co‐transfection with an HNF1α expression vector demonstrates a direct activation of the promoter by HNF1α through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco‐2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.