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Cloning and expression of the bovine sodium‐ and chloride‐dependent noradrenaline transporter
Author(s) -
Lingen Bettina,
Brüss Michael,
Bönisch Heinz
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80508-3
Subject(s) - transporter , complementary dna , cdna library , amino acid , biochemistry , sodium , microbiology and biotechnology , chemistry , peptide sequence , cloning (programming) , molecular cloning , biology , gene , organic chemistry , computer science , programming language
A cDNA encoding a functional bovine, tricyclic antidepressant‐sensitive noradrenaline transporter has been identified by screening a λgt11 cDNA library of the bovine adrenal medulla using the cDNA of the human noradrenaline transporter [1991, Nature 350, 350‐354]. The sequence predicts a protein of 615 amino acids ( M r , 68 900). The bovine transporter shares 93% amino acid identity with the human sequence, but displays two more consensus sites for phosphorylation by protein kinase C. Transient expression of the transporter in COS‐7 cells resulted in a sodium‐ and chloride‐dependent uptake of noradrenaline with a pharmacology typical for a neuronal noradrenaline transporter.