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Synthesis and characterization of sapecin and sapecin B
Author(s) -
Il Kim Jae,
Iwai Hideo,
Kurata Shoichiro,
Takahashi Masami,
Masuda Katuyoshi,
Shimada Ichio,
Natori Shunji,
Arata Yoji,
Sato Kazuki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80498-2
Subject(s) - characterization (materials science) , chemistry , computational biology , biology , nanotechnology , materials science
Two insect defencins, sapecin and sapecin B, were chemically synthesized to confirm their structure and antibacterial activity and also to examine the possibility that these peptides bind to the same site on the large conductance calcium‐activated potassium channel as charybdotoxin. Both synthetic peptides showed the same antibacterial activity as native sapecins, indicating that the synthetic peptides folded correctly in the chemical synthesis. Synthetic sapecins did not show an inhibitory effect on [ 125 I]charybdotoxin binding to rat brain synaptic membranes, suggesting that sapecin B recognizes a different binding site from that of charybdotoxin despite the similar structural motif.