Premium Biochemical properties of attachment region binding protein ARBPPremium
Author(s)
von Kries Jens P.,
Rosorius Olaf,
Buhrmester Hartmut,
Strätling Wolf H.
Publication year1994
Publication title
febs letters
Resource typeJournals
PublisherWiley-Blackwell
ARBP (attachment region binding protein) is an abundant nuclear protein that specifically binds to matrix/scaffold attachment regions (MARs/SARs). Here we show by gel filtration and gradient sedimentation that ARBP has an elongated shape. The sedimentation coefficient was determined as only 2.1 S. Furthermore, limited proteolysis of ARBP in situ (in isolated nuclei) with several proteases generated limiting resistant peptides from 14.5 to 18 kDa, that retained the ability to bind MARs specifically. This indicates that these peptides encompass the DNA binding domain of ARBP.
Subject(s)astrobiology , biochemistry , biology , biophysics , chemistry , enzyme , mars exploration program , microbiology and biotechnology , proteases , proteolysis , sedimentation coefficient
Language(s)English
SCImago Journal Rank1.593
H-Index257
eISSN1873-3468
pISSN0014-5793
DOI10.1016/0014-5793(94)80497-4
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