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Biochemical properties of attachment region binding protein ARBP
Author(s) -
von Kries Jens P.,
Rosorius Olaf,
Buhrmester Hartmut,
Strätling Wolf H.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80497-4
Subject(s) - proteolysis , chemistry , biophysics , mars exploration program , proteases , biochemistry , sedimentation coefficient , microbiology and biotechnology , biology , enzyme , astrobiology
ARBP (attachment region binding protein) is an abundant nuclear protein that specifically binds to matrix/scaffold attachment regions (MARs/SARs). Here we show by gel filtration and gradient sedimentation that ARBP has an elongated shape. The sedimentation coefficient was determined as only 2.1 S. Furthermore, limited proteolysis of ARBP in situ (in isolated nuclei) with several proteases generated limiting resistant peptides from 14.5 to 18 kDa, that retained the ability to bind MARs specifically. This indicates that these peptides encompass the DNA binding domain of ARBP.