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Oxidation of LDL by nitric oxide and its modification by Superoxides in macrophage and cell‐free systems
Author(s) -
Wang Jin-Mei,
Chow Song-Nan,
Lin Jen-Kun
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80494-x
Subject(s) - nitric oxide , chemistry , superoxide dismutase , sodium nitroprusside , catalase , superoxide , biochemistry , macrophage , oxidative stress , enzyme , in vitro , organic chemistry
Oxidative modification of low density lipoproteins (LDL) can increase the atherogenicity of LDL. Here we demonstrate the potential role of nitric oxide (NO) in LDL modification. Dose‐dependent alterations of LDL including an increase in electrophoretic mobility, generation of peroxides and degradation of apoprotein B occurred during incubation with NO. Sodium nitroprusside was also shown to increase the electromobility of LDL. Superoxide dismutase (SOD) and catalase did not affect this result. NO treatment also increases the negative charges on other lipoproteins. In addition, we have demonstrated that NO plays a protective role in macrophage or superoxide‐induced LDL modification through its neutralizing action.