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Molecular cloning of rat JAK3, a novel member of the JAK family of protein tyrosine kinases
Author(s) -
Takahashi Takamune,
Shirasaw Takuji
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80485-0
Subject(s) - tyrosine kinase , cloning (programming) , sh2 domain , receptor tyrosine kinase , kinase , sh3 domain , microbiology and biotechnology , chemistry , biology , biochemistry , signal transduction , computer science , programming language
We have cloned and sequenced a cDNA (JAK3) encoding a novel member of the JAK family of protein tyrosine kinases. JAK3 was identified by RT‐PCR of rat mesangial cells using degenerate oligonucleotide primers, and a full‐length clone was isolated from a rat spleen cDNA library. The primary structure of JAK3 showed cDNA with an open reading frame of 1,100 amino acids which comprises the PTK catalytic domain and a second kinase‐related domain characteristic for JAK kinase. JAK3 was phylogenetically shown to be most closely related to JAK2 among the previously known JAK family members, JAK1, JAK2 and Tyk2. Southern analysis revealed that JAK3 is a single copy gene and well conserved in the vertebral genome. Northern analysis indicated that the 4.0 kb mRNA was transcribed in a variety of tissues including spleen, lung, kidney and intestine.