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Immunoreactive adrenomedullin in human plasma
Author(s) -
Kitamura Kazuo,
Ichiki Yoshinari,
Tanaka Miho,
Kawamoto Mari,
Emura Junji,
Sakakibara Shumpei,
Kangawa Kenji,
Matsuo Hisayuki,
Eto Tanenao
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80474-5
Subject(s) - adrenomedullin , radioimmunoassay , sephadex , endocrinology , medicine , chemistry , size exclusion chromatography , chromatography , peptide , biochemistry , enzyme
A specific and sensitive radioimmunoassay for adrenomedullin has been developed. Half‐maximal inhibition of binding of radioiodinated adrenomedullin was observed at 4 tool/tube. The radioimmunoassay recognized the entire adrenomedullin molecule and has little crossreactivity with adrenomedullin fragment peptides. Adrenomedullin‐like immunoreactivity was found to circulate in human plasma at considerable concentration (3.3 ± 0.39 ). The immunoreactivity of adrenomedullin was eluted at almost the same position as synthetic adrenomedullin on gel‐filtration chromatography and reverse‐phase high‐performance liquid chromatography, suggesting that circulating adrenomedullin recognized by the present radioimmunoassay is identical or very similar to authentic adrenomedullin. Plasma immunoreactive adrenomedullin significantly increased in patients with hypertension, with a progressive rise proportionate to disease severity.