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Aequorea green fluorescent protein
Author(s) -
Inouye Satoshi,
Tsuji Frederick I.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80472-9
Subject(s) - aequorin , green fluorescent protein , fluorescence , aequorea victoria , luminescence , bioluminescence , chemistry , biophysics , energy transfer , biochemistry , biology , materials science , physics , optoelectronics , quantum mechanics , molecular physics , intracellular , gene
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.