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Effect of glutathione depletion on formation of paramagnetic chromium in Chinese hamster V‐79 cells
Author(s) -
Sugiyama Masayasu,
Tsuzuki Katsuyuki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80471-0
Subject(s) - glutathione , buthionine sulfoximine , chemistry , chromium , hamster , intracellular , glutathione reductase , chinese hamster , biochemistry , microbiology and biotechnology , enzyme , biology , glutathione peroxidase , in vitro , organic chemistry
Incubation of Chinese hamster V‐79 cells with either buthionine sulfoximine (BSO) or diethylmaleate (DEM) prior to exposure to Na 2 CrO 4 resulted in a depletion of GSH. ESR study shows that the depletion of GSH by BSO caused an increase in the cellular level of Cr(V) intermediate without affecting the level of Cr(III) complex, whereas the levels of Cr(V) and (III) were both suppressed by the depletion of GSH by DEM. GSH depletion by DEM decreased cellular uptake of chromate more than that by BSO. Glutathione reductase activity in cells, which is capable of reducing Cr(VI), was unaffected by BSO, however a strong inhibition was observed in cells treated with DEM, indicating that DEM affects not only GSH levels but also Cr(VI)‐reducing enzyme activity. Thus, the depletion of GSH in cells increases Cr(V) formation, based upon the effect of BSO. The role of intracellular GSH in Cr(VI) reduction is discussed.