Premium
Yeast cells with a specific cellular make‐up and an environment that removes acetaldehyde are prone to sustained glycolytic oscillations
Author(s) -
Richard Peter,
Diderich Jasper A.,
Bakker Barbara M.,
Teusink Bas,
van Dam Karel,
Westerhoff Hans V.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80461-3
Subject(s) - acetaldehyde , glycolysis , cyanide , hexokinase , biochemistry , chemistry , saccharomyces cerevisiae , respiration , substrate (aquarium) , biophysics , steady state (chemistry) , yeast , ethanol , microbiology and biotechnology , enzyme , biology , inorganic chemistry , anatomy , ecology
Glycolytic oscillations can be induced by adding glucose to starved Saccharomyces cerevisiae cells and, after a steady state has been established, cyanide. Transient oscillations or limit‐cycle oscillations can be induced depending on the growth phase in which the cells are harvested. To find what causes these differences in the dynamic behaviour, we analyzed glycolytic enzyme activities at different growth phases. The hexokinase activity increased by a factor of three after growth substrate transition from glucose to ethanol; the other measured activities remained constant. Cyanide was found not only to block respiration, but also to trap acetaldehyde. Both cyanide actions appear necessary for the occurrence of sustained glycolytic oscillations.