Premium
Radioimmunoassay for circulating human guanylin
Author(s) -
Kuhn Michaela,
Kulaksiz Hasan,
Adermann Knut,
Rechkemmer Gerhurd,
Forssmann Wolf-Georg
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80460-5
Subject(s) - radioimmunoassay , endocrinology , bioassay , medicine , antibody , western blot , chemistry , peptide hormone , biology , hormone , biochemistry , immunology , gene , genetics
A highly specific and sensitive radioimmunoassay for circulating human guanylin (guanylin‐22–115) has been developed. Antibodies were raised against the amino‐terminus (positions 4–16) of the peptide. Western blot analysis confirmed that the antibody selected for radioimmunoassay recognizes circulating high molecular weight (10.3 kDa) guanylin. Extraction and purification of guanylin from blood hemofiltrate and from blood plasma showed that circulating guanylin is detectable in corresponding amounts by the radioimmunoassay and by a specific bioassay. In 30 healthy subjects, the mean plasma concentration of immunoreactive (IR) guanylin was 42 ± 3 . In 22 patients with chronic renal insufficiency, the concentrations of IR‐guanylin were significantly enhanced (1,074 ± 24 ), indicating that kidneys metabolize and/or eliminate the circulating hormone.