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Both interleukin‐8 receptors independently mediate chemotaxis
Author(s) -
Loetscher Pius,
Seitz Michael,
Clark-Lewis Ian,
Baggiolini Marco,
Moser Bernhard
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80454-0
Subject(s) - chemotaxis , receptor , chemokine , jurkat cells , microbiology and biotechnology , transfection , biology , cxc chemokine receptors , cytokine , chemistry , t cell , chemokine receptor , cell culture , immunology , biochemistry , immune system , genetics
Neutrophil leukocytes, the target cells for interleukin‐8 and related CXC chemokines, bear high numbers of two types of IL‐8 receptors (IL‐8R1 and IL‐8R2). By cDNA transfection Jurkat cell lines were generated that stably express either IL‐8R1 or IL‐8R2 (J‐I8L8R1 and J‐IL8R2). J‐IL8R1 expressed 4,000 ± 1,000 copies of IL‐8R 1, and bound IL‐8 with high affinity ( K d 1–4 nM) and GROα and NAP‐2 with low affinity ( K d 200–500 nM). J‐IL8R2 expressed 17,000 ± 3,000 copies of IL‐8R2, and bound all three chemokines with high affinity. Both transfectants showed a similar degree of chemotactic migration after stimulation with IL‐8, GROa and NAP‐2. All three chemokines were equally potent as attractants of J‐IL8R2, whereas IL‐8 was 300 to 1,000‐fold more potent than GROα or NAP‐2 as attractant of J‐IL8R1. The potencies, therefore, agree with the affinities of the ligands to IL‐8R1 and IL‐8R2. Our results demonstrate that both IL‐8 receptors function independently, and mediate chemotaxis in response to IL‐8 and other CXC chemokines.