Premium
cDNA cloning, expression and primary structure of Par j I, a major allergen of Parietaria judaica pollen
Author(s) -
Costa M.A.,
Colombo P.,
Izzo V.,
Kennedy H.,
Venturella S.,
Cocchiara R.,
Mistrello G.,
Falagiani P.,
Geraci D.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80453-2
Subject(s) - complementary dna , allergen , microbiology and biotechnology , open reading frame , cloning (programming) , biology , cdna library , pollen , recombinant dna , peptide sequence , molecular cloning , coding region , nucleic acid sequence , genetics , dna , gene , allergy , immunology , botany , computer science , programming language
A 659 bp cDNA clone 1 coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par j I since it reacted with 95% of the sera from Pj‐allergic patients ( n = 22) and with two Par j I‐specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.