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The myeloid‐lineage specific enhancer of the mouse myeloperoxidase gene consists of three cis ‐elements defined as in vitro DNase I footprints
Author(s) -
Zhu J.D
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80424-9
Subject(s) - enhancer , hypersensitive site , in vitro , microbiology and biotechnology , dnase i hypersensitive site , deoxyribonuclease i , gene , myeloperoxidase , biology , regulatory sequence , myeloid , footprinting , enhancer trap , dna , chemistry , genetics , regulation of gene expression , gene expression , transcription factor , base sequence , immunology , inflammation
The myeloid‐lineage specific enhancer at 3.4–3.1 kb upstream of the mouse myeloperoxidase gene [1] has been further characterised. In vitro DNase I footprinting experiments revealed three protected sequences (FT‐I, ‐II and ‐III) in the enhancer, associated with the proteins that are enriched in WEHI 3BD + cells, at which the MPO gene is highly expressed; but not in two non‐MPO expressing lymphocytic cell lines. Site‐specific mutations at each element severely reduced the level of the reporter gene activity in a non‐additive manner. This is parallel with either abolishment or alteration of the corresponding wild‐type protein‐DNA interaction in vitro. Consideration of the sequence motifs present in the enhancer suggests that the cis ‐elements denned as the in vitro DNase I footprints are likely to be novel.