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Monoclonal non‐specific suppressor factor (MNSF) inhibits the IL4 secretion by bone marrow‐derived mast cell (BMMC)
Author(s) -
Nakamura Morihiko,
Xavier Ricardo M.,
Tanigawa Yoshinori
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80423-0
Subject(s) - secretion , mast cell , bone marrow , interleukin 4 , suppressor , microbiology and biotechnology , biology , immunology , chemistry , endocrinology , cytokine , biochemistry , gene
We investigated whether monoclonal non‐specific suppressor factor (MNSF) has an effect on the IL4 secretion by BMMC sensitized with anti‐DNP IgE monoclonal antibody. The sensitized BMMC showed a maximum increase of IL4 secretion at 10 antigen activation. When MNSF (10 U) was added, it remarkably suppressed the IL4 secretion (82 ± 3%). MNSF also suppressed the IL4 secretion by the mast cell line, CFTL‐15, but not CFTL‐12. Northern blot analysis showed that, when the same amounts of MNSF were added, IL4 mRNA expression was decreased, suggesting that the suppression results from the inhibition of a transcript.