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Identification of a cDNA encoding a Drosophila calcium/calmodulin regulated protein phosphatase, which has its most abundant expression in the early embryo
Author(s) -
Brown Linda,
Chen Mao Xiang,
Cohen Patricia T.W.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80398-6
Subject(s) - complementary dna , calmodulin , biology , microbiology and biotechnology , cdna library , gene isoform , peptide sequence , embryo , phosphatase , calcium binding protein , biochemistry , gene , calcium , enzyme , chemistry , organic chemistry
A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73–78% identical to human PP2B isoforms. The cDNA hybridised to the X‐chromosome at cytological position 14D1‐4. Two transcripts of 3.5 kb and 3.0 kb were expressed during embryonic development, their levels being highest in the early embryo. The larger transcript was also clearly present in adult females. This pattern of expression indicates a role for calcium/calmodulin regulated protein phosphatase in embryonic development.