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Detection of specific glucose‐3‐phosphatase activity in rat liver
Author(s) -
Canales José,
Buitrago Francisco,
Faraldo Angeles,
Cameselle José Carlos
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80383-8
Subject(s) - glucose 6 phosphatase , phosphatase , chemistry , biochemistry , alkaline phosphatase , enzyme
Sugar‐3‐phosphates are related to aspects of diabetes which depend on protein glycosylation events. Sorbitol‐3‐phosphate and fructose‐3‐phosphate occur in normal and diabetic individuals, and glucose‐3‐phosphate is a potential intermediate in their biosynthesis. Almost nothing is known about enzyme pathways for their metabolic turnover. We have found that part of the phosphohydrolytic activity on glucose‐3‐phosphate in rat liver supernatants corresponds to a specific, Mg 2+ ‐dependent, glucose‐3‐phosphatase much less or not active on other phosphate esters, including glucose‐1‐phosphate, glucose‐6‐phosphate, fructose‐1‐phosphate, fructose‐6‐phosphate and p ‐nitrophenyl‐phosphate. This finding opens a route to a better understanding of the metabolism and role of sugar‐3‐phosphates.