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Site‐specific mutagenesis of the reaction centre from Rhodobacter sphaeroides studied by Fourier transform Raman spectroscopy: mutations at tyrosine M210 do not affect the electronic structure of the primary donor
Author(s) -
Jones Michael R.,
Heer-Dawson Manuel,
Mattioli Tony A.,
Hunter C.Neil,
Robert Bruno
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80376-5
Subject(s) - rhodobacter sphaeroides , bacteriochlorophyll , chemistry , photosynthetic reaction centre , electron transfer , tyrosine , photochemistry , raman spectroscopy , spectroscopy , stereochemistry , biochemistry , photosynthesis , optics , physics , quantum mechanics
The effects of mutation of residue tyrosine M210 on the primary donor bacteriochlorophylls have been investigated by near infrared FT‐Raman spectroscopy in reaction centres purified from an antenna‐deficient strain of Rhodobacter sphaeroides . We find that mutation at the M210 position does not significantly perturb the distribution of the unpaired electron over the pair of bacteriochlorophyll molecules which constitute the primary donor radical cation. We conclude, therefore, that the effects of mutation of tyrosine M210 on the rate and asymmetry of primary electron transfer in reaction centres cannot be ascribed to a change in the electronic structure of the primary donor.