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Enhancement of hydrogen peroxide‐induced luminol‐dependent chemiluminescence by neopterin depends on the presence of iron chelator complexes
Author(s) -
Murr Christian,
Fuchs Dietmar,
Gössler Walter,
Hausen Arno,
Reibnegger Gilbert,
Werner Ernst R.,
Werner-Felmayer Gabriele,
Esterbauer Hermann,
Wachter Helmut
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80369-2
Subject(s) - neopterin , hydrogen peroxide , chemiluminescence , chemistry , luminol , chelation , penicillamine , reactive oxygen species , biochemistry , inorganic chemistry , immunology , chromatography , organic chemistry , biology
We have previously shown that neopterin, 6‐ d ‐erythro‐trihydroxypropyl‐pteridine, synthesized by human monocytes/macrophages upon stimulation by interferon‐γ, enhances toxicity of reactive oxygen at neutral or slightly alkaline pH (7.5), but not at acidic pH (below 6.5). In the present study, we explored in more detail the necessary requirements for neopterin to modulate the effects of hydrogen peroxide in a luminol‐dependent chemiluminescence assay. We demonstrate that neopterin enhances hydrogen peroxide effects only in the presence of iron chelator complexes like iron‐(III)‐ or iron‐(II)‐EDTA or iron‐(III)‐DTPA. Thus, iron chelator complexes together with neopterin may play an important role in macrophage‐mediated effector mechanisms.