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Human μ opiate receptor
Author(s) -
Wang Jia-Bei,
Johnson Peter S.,
Persico Antonio M.,
Hawkins Anita L.,
Griffin Constance A.,
Uhl George R.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80368-4
Subject(s) - complementary dna , opiate , cdna library , biology , in situ hybridization , microbiology and biotechnology , receptor , gene , genetics , gene expression
A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium‐ and GTP‐sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24–25. An Msp I polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.