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Different induction mechanisms of mRNA for inducible nitric oxide synthase in rat smooth muscle cells in culture and in aortic strips
Author(s) -
Sirsjo Allan,
Söderkvist Peter,
Sundqvist Tommy,
Carlsson Maria,
Öst Martin,
Gidlöf Anders
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80363-3
Subject(s) - nitric oxide synthase , messenger rna , smooth muscle , nitric oxide , chemistry , microbiology and biotechnology , anatomy , biochemistry , biology , endocrinology , gene
The expression of mRNA for the inducible form of nitric oxide synthase, (iNOS), was studied in rat aortic smooth muscle cells, (SMCs) in cell culture and in strips of rat aorta by reverse transcriptase coupled to the polymerase chain reaction. iNOS mRNA expression was weak in cultured SMCs when exposed to either interferon‐γ (IFN γ ) or lipopolysaccharide (LPS), but the combination LPS + IFN γ enhanced the expression. In aortic strips LPS alone induced a pronounced expression, with no further increase by IFN γ . Cycloheximide potentiated the expression of iNOS mRNA in SMCs in culture stimulated with LPS + IFN γ but attenuated the response in aortic strips.The results indicate different cellular signaling pathways for the induction of iNOS mRNA by LPS and/or IFN γ , in cultured SMCs and in rat aortic strips.