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Analysis of an insulin gene transcription control element
Author(s) -
Stein Roland,
Henderson Eva,
Cordle Susan R.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80362-5
Subject(s) - enhancer , transcription (linguistics) , response element , biology , gene , psychological repression , e box , transcription factor , negative regulatory element , insulin , gene expression , regulatory sequence , microbiology and biotechnology , genetics , promoter , endocrinology , linguistics , philosophy
Pancreatic β‐cell‐type‐specific transcription of the insulin gene is controlled by cis ‐acting sequence elements lying within its enhancer region. An essential element required for expression is the insulin control element (ICE). The activity of this element is regulated by both positive‐ and negative‐acting transcription factors. In this study, we have identified the nucleotide sequences within the ICE that are required for repression in noninsulin producing cells. Our results indicate that the cis ‐acting sequences involved in negative control are distinct from those required in activating expression in β cells.