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On the tertiary structure of the Citrus ichangensis satellite DNA
Author(s) -
Beridze T.,
Tsirekidze N.,
Turishcheva M.S.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80360-9
Subject(s) - random hexamer , satellite dna , dna , satellite , gel electrophoresis , solenoid , chemistry , polyacrylamide gel electrophoresis , helix (gastropod) , crystallography , biology , physics , biochemistry , chromosome , enzyme , gene , paleontology , quantum mechanics , astronomy , snail
A hexamer of the repeating unit of Citrus ichangensis satellite DNA was cloned. Polyacrylamide gel electrophoresis demonstrated that the shape of cloned hexamer is other than linear. As the computing of tertiary coordinates made by Eckdahl and Anderson's BEN program proved, the hexamer is a solenoid consisting of two turns termed coiled double helix (CDH)‐form. An electron microscopic analysis revealed small diameter circles in the hexamer under investigation. Unlike the hexamer control molecules are s‐shaped. It is concluded that the CDH‐form is a characteristic of the satellite DNA of Citrus ichangensis .