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Level of ATP synthase activity required for yeast Saccharomyces cerevisiae to grow on glycerol media
Author(s) -
Mukhopadhyay Arindam,
Uh Misook,
Mueller David M.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80310-2
Subject(s) - saccharomyces cerevisiae , yeast , glycerol , atp synthase , biochemistry , chemistry , enzyme
Two independent cold‐sensitive pet mutants in the gene (ATP5) coding for the oligomycin sensitivity conferring protein (OSCP) have been isolated in the yeast Saccharomyces cerevisiae . The mutations in both strains alter the initiating methionine codon in the ATP5 gene: ATG to ATA (Ile) and AAG (Lys). Western blot analysis of total yeast protein after the cells were grown at 18°C, 30°C, and 37°C, indicates that the level of OSCP decreased 80% relative to the wild type strain. In addition, the level of the oligomycin‐sensitive ATPase decreased 85% relative to the wild type strain, after growth at 30°C. These findings indicate that for S. cerevisiae , the level of oxidative phosphorylation can decrease 85% without showing a large growth defect on media containing glycerol at 30°C, but not at 18°C.