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Molecular cloning of the canine angiotensin II receptor
Author(s) -
Burns Louise,
Clark Kenneth L.,
Bradley John,
Robertson Mark J.,
Clark Adrian J.L.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80307-2
Subject(s) - angiotensin ii , angiotensin ii receptor type 1 , receptor , complementary dna , peptide sequence , transmembrane domain , threonine , biology , microbiology and biotechnology , chemistry , biochemistry , gene , phosphorylation , serine
Canine aortic strip studies revealed insensitivity of angiotensin II (AII)‐induced aortic contraction to inhibition by the non‐peptide antagonist DuP753 (p K B = 6.7 ± 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT 1 AII receptor. Expression of this cDNA in COS‐7 cells indicated a low affinity of DuP753 for the cloned receptor ( K D = 92 nM). The predicted amino acid sequence is highly homologous to other mammalian AT 1 receptors; sequence comparisons suggest the pharmacological difference may be the result of a threonine residue in position 163 in the IVth transmembrane domain.