Premium
Induction of c‐fos gene expression by urokinase‐type plasminogen activator in human ovarian cancer cells
Author(s) -
Dumler I.,
Petri T.,
Schleuning W.-D.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80298-x
Subject(s) - plasminogen activator , urokinase , ovarian cancer , cancer research , gene , activator (genetics) , microbiology and biotechnology , chemistry , biology , cancer , endocrinology , genetics , biochemistry
Binding of urokinase‐type plasminogen activator (u‐PA) to u‐PA receptor (u‐PAR) induces the rapid and transient expression of c‐fos in OC‐7 ovarian carcinoma cells. The pretreatment of the cells with protein tyrosine kinase (PTK) inhibitors, but not the inactivation of the u‐PA active site by DFP (diisopropyl fluorophosphate), abrogates this effect. A soluble u‐PAR fragment, expressed in baculovirus‐infected Sf9 cells and purified by affinity chromatography, competes for binding of u‐PA to u‐PAR and inhibits c‐fos induction. We conclude that activation of u‐PAR after interaction with u‐PA at the cell surface initiates a transmembrane signal, most likely in conjunction with other still unknown protein(s). This signal generates PTK activity feeding into a signal transduction pathway which activates nuclear transcription factors.